📘 ❞ Peptide Nucleic Acids Methods and Protocols ❝ كتاب ــ Peter E. Nielsen اصدار 2002
Biology Books - 📖 كتاب ❞ Peptide Nucleic Acids Methods and Protocols ❝ ــ Peter E. Nielsen 📖
█ _ Peter E Nielsen 2002 حصريا كتاب ❞ Peptide Nucleic Acids Methods and Protocols ❝ 2024 Protocols: نبذه عن الكتاب: PNA Technology Peter Nielsen 1 Introduction Peptide nucleic acids (PNA) were originally conceived and designed as sequence specific DNA binding reagents targeting the DNA major groove in analogy to triplex forming oligonucleotides However, instead of the sugar phosphate backbone PNA was designed with a pseudopeptide (1) Once synthesized, it apparent that oligomers based on the aminoethylglycin acetyl linkers nucleobases (see Fig 1) are extremely good structural mimics (or RNA), being able form very stable duplex structures with Watson Crick complementary DNA, RNA (or PNA) (2– 4) It also quickly became clear triplexes formed between one homopurine RNA) strand two strands extraordinarily Furthermore, this stability is reason why homopyrimidine when binding targets double stranded do not do so by conventional (PNA DNA2) formation, but rather prefer invasion complex which is invaded an internal PNA2 (see 2) (5,6) This type restricted homopurine DNA targets full dsDNA oligo Biology Books مجاناً PDF اونلاين Biologically Biology natural science concerned study life, its various forms function, how these organisms interact each other surrounding environment The word biology Greek made up words: bio (βίος) meaning life And loggia ( λογία) means or Biology: similarity vegetation animal cover edges African American states, existence same fossil Branches biology Biology ancient thousands years old modern began nineteenth century This has multiple branches Among them are: Anatomy Botany Biochemia Biogeography Biofisia Cytology cell science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology functions organs Taxonemia taxonomy Virology virology Zoology zoology
كتاب
Peptide Nucleic Acids Methods and Protocols
ــ Peter E. Nielsen
صدر 2002م
كتاب
Peptide Nucleic Acids Methods and Protocols
ــ Peter E. Nielsen
صدر 2002منبذه عن الكتاب:
PNA Technology
Peter E. Nielsen
1. Introduction
Peptide nucleic acids (PNA) were originally conceived and
designed as sequence-specific DNA binding reagents targeting the
DNA major groove in analogy to triplex-forming oligonucleotides.
However, instead of the sugar-phosphate backbone of oligonucleotides PNA was designed with a pseudopeptide backbone (1). Once
synthesized, it was apparent that PNA oligomers based on the
aminoethylglycin backbone with acetyl linkers to the nucleobases
(see Fig. 1) are extremely good structural mimics of DNA (or
RNA), being able to form very stable duplex structures with
Watson-Crick complementary DNA, RNA (or PNA) oligomers (2–
4). It also quickly became clear that triplexes formed between one
homopurine DNA (or RNA) strand and two sequence complementary PNA strands are extraordinarily stable. Furthermore, this stability is the reason why homopyrimidine PNA oligomers when
binding complementary targets in double-stranded DNA do not do
so by conventional (PNA-DNA2) triplex formation, but rather prefer to form a triplex-invasion complex in which the DNA duplex is
invaded by an internal PNA2-DNA triplex (see Fig. 2) (5,6). This
type of binding is restricted to homopurine/homopyrimidine DNA
targets in full analogy to dsDNA targeting by triplex forming oligo
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