📘 ❞ Protein Arrays Methods and Protocols ❝ كتاب ــ Eric T. Fung, MD, PhD اصدار 2004
Biology Books - 📖 كتاب ❞ Protein Arrays Methods and Protocols ❝ ــ Eric T. Fung, MD, PhD 📖
█ _ Eric T Fung, MD, PhD 2004 حصريا كتاب ❞ Protein Arrays Methods and Protocols ❝ 2024 Protocols: نبذه عن الكتاب: Protein From cDNA Expression Libraries Hendrik Weiner, Thomas Faupel, Konrad Büssow Summary This chapter describes the production of a expression library from human fetal brain, the construction high density protein array such library, two applications to screen for binding proteins After producing decollating expression clones, one can pick thousands clones with laboratory robot deposit them into microtiter plates in an ordered manner Such clone libraries are starting material This is constructed by spotting onto membrane Following cell growth induction on membrane, spots lysed their recombinant protein immobilized The so carries proteins without need to clone, express, spot individual arrays allow screen for numerous functions throughput Key Words: Protein array; library; spotting; antigen; protein function; protein–protein interaction; posttranslational modification; throughput screening 1 Introduction Arrays complementary DNA (cDNA) carry of proteins without (1) These arrays practical formats screen en masse given function, that is, to identify antigens (1,2), including autoantigens (3), (4), and substrates arginine methyltransferases (5) Although not yet demonstrated, the arrays may also permit studies modifications other than protein methylation, find substrates certain kinases The described here made using vectors With help robot, deposit them Biology Books مجاناً PDF اونلاين Biologically Biology natural science concerned study life, its various forms how these organisms interact each surrounding environment word biology Greek up words: bio (βίος) meaning life And loggia ( λογία) means or Biology: similarity vegetation animal cover edges African American states, existence same fossil Branches biology Biology ancient years old modern began nineteenth century has multiple branches Among are: Anatomy Botany Biochemia Biogeography Biofisia Cytology science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology organs Taxonemia taxonomy Virology virology Zoology zoology
كتاب
Protein Arrays Methods and Protocols
ــ Eric T. Fung, MD, PhD
صدر 2004م كتاب
Protein Arrays Methods and Protocols
ــ Eric T. Fung, MD, PhD
صدر 2004م
نبذه عن الكتاب:
Protein Arrays From cDNA Expression Libraries
Hendrik Weiner, Thomas Faupel, and Konrad Büssow
Summary
This chapter describes the production of a cDNA expression library from human fetal brain,
the construction of a high-density protein array from such a library, and two applications to
screen the array for binding proteins. After producing the library and decollating the expression
clones, one can pick thousands of expression clones with a laboratory robot and can deposit
them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting
material for the construction of a high-density protein array. This array is constructed by spotting the expression clones onto a protein-binding membrane. Following cell growth and induction of protein expression on the membrane, the cell spots are lysed and their recombinant
protein immobilized on the membrane. The so-constructed array carries thousands of proteins
without the need to clone, express, and spot individual proteins. Such arrays allow one to screen
for numerous protein functions in a high-throughput manner.
Key Words:
Protein array; cDNA expression library; high-density spotting; clone array; protein antigen;
protein function; protein–protein interaction; posttranslational modification; high-throughput
screening.
proteins without the need to clone, express, and spot individual proteins (1). These
arrays are practical formats to screen en masse for a given protein function, that is, to
identify protein antigens (1,2), including autoantigens (3), binding proteins (4), and
substrates for arginine methyltransferases (5). Although not yet demonstrated, the
arrays may also permit studies on posttranslational modifications other than protein
methylation, that is, to find substrates for certain protein kinases.
The protein arrays described here are made using cDNA libraries that are constructed in expression vectors. With the help of a laboratory robot, one can pick thousands of library clones and can deposit them into microtiter plates in an ordered
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