📘 ❞ Plant Functional Genomics. Methods and Protocols ❝ كتاب ــ Erich Grotewold اصدار 1996
Biology Books - 📖 كتاب ❞ Plant Functional Genomics. Methods and Protocols ❝ ــ Erich Grotewold 📖
█ _ Erich Grotewold 1996 حصريا كتاب ❞ Plant Functional Genomics Methods and Protocols ❝ 2025 Protocols: نبذه عن الكتاب: An Improved Method for BAC Library Construction Meizhong Luo Rod A Wing Summary Large genomic DNA insert containing libraries are required as critical tools physical mapping, positional cloning, genome sequencing of complex genomes The bacterial artificial chromosome (BAC) cloning system has become a dominant over others to clone large inserts As the costs physical mapping, genome sequencing continuously decrease, there is an increasing demand high quality deepcoverage large BAC In our laboratory, we have constructed many quality deep coverage including arabidopsis, manocot dicot crop plants, and plant pathogens Here, present protocol used in laboratory construct BAC libraries Key Words BAC, library, method, pCUGIBAC1, plant 1 Introduction Large essential There two principal systems that as yeast or chromosomes (YACs BACs, respectively) The YAC (1) was first developed 1987 uses Saccharomyces cerevisiae host maintains (up 1 Mb) linear molecules with pair yeast telomeres centromere Although extensively late 1980s early 1990s, this several disadvantages (2,3) recombinant can be unstable manipulation is difficult inefficient Most importantly, level chimerism, clon Biology Books مجاناً PDF اونلاين Biologically Biology natural science concerned study life, its various forms function, how these organisms interact each other surrounding environment word biology Greek made up words: bio (βίος) meaning life And loggia ( λογία) means Biology: similarity vegetation animal cover on edges African American states, existence same fossil Branches biology Biology ancient thousands years old modern began nineteenth century This multiple branches Among them are: Anatomy Botany Biochemia Biogeography Biofisia Cytology cell science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology functions organs Taxonemia taxonomy Virology virology Zoology zoology
كتاب
Plant Functional Genomics. Methods and Protocols
ــ Erich Grotewold
صدر 1996م كتاب
Plant Functional Genomics. Methods and Protocols
ــ Erich Grotewold
صدر 1996م
نبذه عن الكتاب:
An Improved Method for Plant BAC
Library Construction
Meizhong Luo and Rod A. Wing
Summary
Large genomic DNA insert-containing libraries are required as critical tools for physical
mapping, positional cloning, and genome sequencing of complex genomes. The bacterial artificial chromosome (BAC) cloning system has become a dominant system over others to clone
large genomic DNA inserts. As the costs of positional cloning, physical mapping, and genome
sequencing continuously decrease, there is an increasing demand for high-quality deepcoverage large insert BAC libraries. In our laboratory, we have constructed many high-quality
deep-coverage large insert BAC libraries including arabidopsis, manocot and dicot crop plants,
and plant pathogens. Here, we present the protocol used in our laboratory to construct BAC
libraries.
Key Words
BAC, library, method, pCUGIBAC1, plant
mapping, positional cloning, and genome sequencing of complex genomes.
There are two principal large insert cloning systems that are constructed as
yeast or bacterial artificial chromosomes (YACs and BACs, respectively). The
YAC cloning (1) was first developed in 1987 and uses Saccharomyces
cerevisiae as the host and maintains large inserts (up to 1 Mb) as linear molecules with a pair of yeast telomeres and a centromere. Although used extensively in the late 1980s and early 1990s, this system has several disadvantages
(2,3). The recombinant DNA in yeast can be unstable. DNA manipulation is
difficult and inefficient. Most importantly, a high level of chimerism, the clon-
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