📘 ❞ Principles and Protocols-Humana Press ❝ كتاب ــ Marilena Aquino de Muro Ralph Rapley اصدار 2001
Biology Books - 📖 كتاب ❞ Principles and Protocols-Humana Press ❝ ــ Marilena Aquino de Muro Ralph Rapley 📖
█ _ Marilena Aquino de Muro Ralph Rapley 2001 حصريا كتاب ❞ Principles and Protocols Humana Press ❝ 2024 Press: نبذه عن الكتاب: The isolation of specific regions within the genome an organism is now normally accomplished by polymerase chain reaction (PCR) amplification using primers for region in question However, there are occasions where this not possible (loss primer sites resulting no amplification or if there available) In these cases detection sequence of interest can be achieved hybridization a labeled probe to restricted genomic DNA immobilized on membrane Southern blotting (1) Genomic DNA first digested one more restriction enzymes fragments generated separated gel electrophoresis The amount applied to varies from application general 10 µg human genomic needed single copy gene when using radioactively probes overnight exposure X ray film This figure reduced if target either repetitive element (e g , ribosomal DNA) or, plasmid PCR products run Once fragments then denatured situ transferred capillary transfer nitrocellulose nylon The DNA bound membrane, which used a hybridization 1 2 Materials 1 1 Specific 3MM filter paper (Whatman), towels, glass plastic tray support (a gel casting turned upside down), cling Biology Books مجاناً PDF اونلاين Biologically Biology natural science that concerned with study life, its various forms function, how organisms interact each other surrounding environment word biology Greek made up two words: bio (βίος) meaning life And loggia ( λογία) means Biology: similarity vegetation animal cover edges African American states, existence same fossil Branches biology Biology ancient thousands years old modern began nineteenth century This has multiple branches Among them are: Anatomy Botany Biochemia Biogeography Biofisia Cytology cell science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology functions organs Taxonemia taxonomy Virology virology Zoology zoology
كتاب
Principles and Protocols-Humana Press
ــ Marilena Aquino de Muro Ralph Rapley
صدر 2001م كتاب
Principles and Protocols-Humana Press
ــ Marilena Aquino de Muro Ralph Rapley
صدر 2001منبذه عن الكتاب:
The isolation of specific regions within the genome of an organism is now
normally accomplished by polymerase chain reaction (PCR) amplification
using primers specific for the region in question. However, there are occasions
where this is not possible (loss of primer sites resulting in no amplification or if
there are no primers available). In these cases the detection of the sequence of
interest can be achieved by hybridization of a labeled probe to restricted
genomic DNA immobilized on a membrane by Southern blotting (1). Genomic
DNA is first digested by one or more restriction enzymes and the fragments
generated separated by gel electrophoresis. The amount of DNA to be applied
to the gel varies from application to application. In general 10 µg of human
genomic DNA is needed for the detection of a single copy gene when using
radioactively labeled probes and an overnight exposure to X-ray film. This
figure can be reduced if the target is either a repetitive element (e.g., ribosomal
DNA) or, if plasmid DNA or PCR products are run on the gel. Once the fragments are separated on the gel the DNA is then denatured in situ and transferred by capillary transfer to either a nitrocellulose or nylon membrane. The
DNA fragments are then bound to the membrane, which can then be used in a
hybridization reaction.
1.2. Materials
1.2.1. Specific Materials
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