📘 ❞ Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures ❝ كتاب
كتب طب بيطرى - 📖 كتاب ❞ Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures ❝ 📖
█ _ 0 حصريا كتاب ❞ Flow cytometric evaluation of selected antimicrobial efficacy for clearance Anaplasma marginale in short term erythrocyte cultures ❝ 2025 cultures: shortterm من كتب طب بيطرى Flow clearance of Anaplasma marginale in cultures J F COETZEE* M D APLEY*, K M KOCAN & D E JONES à * Department Veterinary Diagnostic and Production Animal Medicine, College Medicine, Iowa State University, Ames, IA, USA; Department of Pathobiology, Center Health Sciences, Oklahoma University, Stillwater, OK, USA; à Department of Veterinary Pathology, College Veterinary Medicine, Iowa University, Ames, IA, USA Coetzee, J , Apley, Kocan, K Jones, evalu ation of Anaplasma marginale in short J vet Pharmacol Therap 29, 173–183 The tick borne rickettsia, Anaplasma marginale , causes the economically important cattle disease anaplasmosis Once infected, remain lifelong carriers Herein, we used flow cytometry to test three antimicrobials; oxytetracycline, imidocarb and enrofloxacin against Virginia (VGN) or (OK) A marginale isolates erythrocyte cultures Parasite viability was assessed using vital dye hydroethidine (HE), which is detectable when living organisms convert HE ethidium bromide Viability of A determined by subinoculation into susceptible calves Data were analyzed MANOVA, Tukey Kramer honest significant difference Wilcoxon rank sum tests Receiver operating characteristic (ROC) analysis correlate results with culture infectivity Enrofloxacin inhibited A a dose dependent manner Surprisingly, higher concentrations less effective than lower concentrations against A marginale with significant differences ( P < 05) observed between two isolates Oxytetracycline least active drug tested Cultures infected OK isolate exposed 4 0 l g mL enrofloxacin and those VGN 1 were sterilized This first in vitro study demonstrating of enrofloxacin marginale Furthermore, these data indicate that flow cytometry useful assay screening antimicrobials (Paper received 16 November 2005; accepted publication 17 February 2006) Hans Coetzee, Department Clinical Sciences, Medicine, Kansas Manhattan, KS 66506–5601, USA mail: jcoetzee@ vet ksu ed مجاناً PDF اونلاين الطب البيطري (بالإنجليزية: medicine) أو البيطرة هو تطبيق المبادئ الطبية والتشخيصية والعلاجية الحيوانات الإنتاجية والمنزلية والبرية يحتوي هذا القسم علي العديد الكتب المتميزة حول المجال يمارس عادة عيادة بيطرية مستشفى بيطري المزرعة للطب دور كبير حماية البشر الأمراض التي تنتقل عن طريق الأكل أصبح التخصص شائعاً السنوات الأخيرة ومن تلك التخصصات: التخدير علم السلوك الجلدية الحالات الطارئة والعناية الحثيثة الباطني امراض القلب السرطان العيون الأعصاب المشتركة المعدية التناسليات والولادة التصوير الشعاعي والجراحة
كتاب
Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures
كتاب
Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures
Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in shortterm erythrocyte cultures من كتب طب بيطرى
Flow cytometric evaluation of selected antimicrobial efficacy for clearance
of
Anaplasma marginale
in short-term erythrocyte cultures
J. F. COETZEE*
M. D. APLEY*,
K. M. KOCAN
&
D. E. JONES
à
and Production Animal Medicine,
College of Veterinary Medicine,
Iowa State University,
Ames, IA, USA;
Department of
Pathobiology, Center for Veterinary Health
Sciences, Oklahoma State University,
Stillwater, OK, USA;
à
Department of
Veterinary Pathology, College of Veterinary
Medicine, Iowa State University, Ames,
IA, USA
Coetzee, J. F., Apley, M. D., Kocan, K. M., Jones, D. E. Flow cytometric evalu-
ation of selected antimicrobial efficacy for clearance of
Anaplasma marginale
in
short-term erythrocyte cultures.
J. vet. Pharmacol. Therap.
29,
173–183.
The tick-borne rickettsia,
Anaplasma marginale
, causes the economically
important cattle disease anaplasmosis. Once infected, cattle remain lifelong
carriers. Herein, we used flow cytometry to test the efficacy of three
antimicrobials; oxytetracycline, imidocarb and enrofloxacin against Virginia
(VGN) or Oklahoma (OK)
A. marginale
isolates in short-term erythrocyte
cultures. Parasite viability was assessed using the vital dye hydroethidine (HE),
which is detectable when living organisms convert HE to ethidium bromide.
Viability of
A. marginale
in selected cultures was determined by subinoculation
into susceptible calves. Data were analyzed by MANOVA, Tukey-Kramer honest
significant difference and Wilcoxon rank sum tests. Receiver operating
characteristic (ROC) analysis was used to correlate results with culture
infectivity. Enrofloxacin inhibited
A. marginale
in a dose dependent manner.
Surprisingly, higher concentrations of imidocarb were less effective than lower
concentrations against
A. marginale
with significant differences (
P
< 0.05)
observed between the two isolates. Oxytetracycline was the least active drug
tested. Cultures infected with the OK isolate exposed to 4.0
l
g/mL enrofloxacin
and those of the VGN and OK isolates exposed to 1.0
l
g/mL imidocarb were
sterilized. This is the first
in vitro
study demonstrating the efficacy of
enrofloxacin against
A. marginale
. Furthermore, these data indicate that flow
cytometry is a useful assay for screening antimicrobials against
A. marginale
.
(Paper received 16 November 2005; accepted for publication 17 February
2006)
Hans Coetzee, Department of Clinical Sciences, College of Veterinary Medicine,
Kansas State University, Manhattan, KS 66506–5601, USA. E-mail: jcoetzee@
vet.ksu.ed
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